Horseradish peroxidase (HRP) is a heme-containing oxidoreductase extracted from horseradish roots. It catalyzes substrate oxidation using hydrogen peroxide. When conjugated to antibodies or other recognition molecules, HRP serves as a reporter enzyme in ELISA, Western blotting, and immunohistochemistry.[1,2] NebuSelect™ Peroxidase from horseradish (Cat. No. NBL-230198) features a specified enzyme activity of ≥310 U/mg, together with an RZ value of ≥3.0 and an approximate molecular mass of 44 kDa. Its defined activity per unit mass provides a reference for enzyme selection and loading design in antibody labeling, immunoassay reagent development, and biochemical reaction systems. Available in 100 μg, 500 μg, and 1 mg sizes, the product supports small-scale evaluation and method development. Please consult the batch-specific Certificate of Analysis or product documentation for activity unit definitions and assay conditions. For research use only. 

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▋References

1.Spadiut O, Herwig C. Production and purification of the multifunctional enzyme horseradish peroxidase. Pharmaceutical Bioprocessing. 2013;1(3):283–295. DOI：10.4155/pbp.13.23 

2.Krainer FW, Glieder A. An updated view on horseradish peroxidases: recombinant production and biotechnological applications. Applied Microbiology and Biotechnology. 2015;99(4):1611–1625. DOI：10.1007/s00253-014-6346-7