NebuSelect™ Anti-Human Serum Rabbit Antiserum (Cat. No. NBAB-160272) is an unconjugated rabbit polyclonal IgG preparation generated by immunizing rabbits with pooled normal human serum, followed by Protein A/G affinity purification. The product is suitable for immunoassays, immunohistochemistry (IHC), and immunofluorescence assays (IFA) for the detection of human serum-associated antigens. For tissue and cell staining, appropriate enzyme- or fluorophore-conjugated anti-rabbit IgG secondary antibodies can be used to visualize target antigens through chromogenic or fluorescent detection.[1,2]

The product is formulated in phosphate-buffered saline (PBS) containing glycerol and sodium azide and is available in 1 mL and 2 mL sizes to accommodate different experimental needs. Storage at 4°C is recommended for short-term use and at −20°C for long-term storage. Avoid repeated freeze–thaw cycles. Working concentrations, sample fixation, and blocking conditions should be optimized in preliminary experiments, with appropriate controls included to assess signal and background.[1,3] For research use only.

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▋References

1.Ramos-Vara JA. Technical aspects of immunohistochemistry. Veterinary Pathology. 2005;42(4):405–426. DOI：10.1354/vp.42-4-405 

2.Im K, et al. An Introduction to Performing Immunofluorescence Staining. Methods in Molecular Biology. 2019;1897:299–311. DOI：10.1007/978-1-4939-8935-5_26 

3.Uhlen M, et al. A proposal for validation of antibodies. Nature Methods. 2016;13(10):823–827. DOI：10.1038/nmeth.3995