At Nebulabio, we offer a wide selection of high-quality Primary Antibodies, Secondary Antibodies, and Control Antibodies designed to support groundbreaking research in molecular biology, immunology, and biomedical sciences. With over 40,000 primary antibodies targeting more than 9,000 proteins, our comprehensive product range is carefully curated to meet the needs of diverse experimental techniques. Our antibodies are rigorously validated for various applications, ensuring reliable, reproducible results across Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FC), and Immunofluorescence(IF), among others.

Our antibodies enable accurate protein detection, quantification, and localization, helping researchers explore cellular processes, signaling pathways, protein interactions, and disease mechanisms. Whether you are studying immune responses, protein modifications, or disease biomarkers, Nebulabio provides the tools necessary for achieving precise experimental outcomes.

 Primary Antibodies                                                 Secondary Antibodies                                                   Control Antibodies

▌Primary Antibodies

Primary Antibodies are designed to bind directly to a specific target protein, allowing researchers to explore various biological pathways and protein functions with high specificity. Our collection includes antibodies targeting a broad array of proteins such as cytokines, adhesion molecules, growth factors, signal transduction proteins, and structural proteins. These antibodies are suitable for applications ranging from cellular analysis to tissue-based assays.

For example, our primary antibodies targeting β-Tubulin, COX IV, GAPDH, NF-κB, and p53 are extensively validated for use in Western Blotting (WB) and Immunohistochemistry (IHC). These antibodies play a pivotal role in studies focused on protein expression, post-translational modifications, and the examination of disease-related pathways. With over 40,000 primary antibodies in our catalog, researchers can find solutions for nearly any target protein, from well-known markers like β-Actin to more specialized targets in cancer research, neurobiology, and stem cell biology.

Our Primary Antibodies are available in various formats, including monoclonal and polyclonal options, ensuring broad compatibility with different experimental setups. Additionally, we offer a wide range of species-specific primary antibodies, making it easier for researchers to use antibodies for their specific model organisms or cell lines.

▌Secondary Antibodies

Secondary Antibodies are indispensable for amplifying the signal from primary antibodies, enhancing the sensitivity of detection in various immunoassay techniques. These antibodies are designed to bind to the Fc region of primary antibodies, enabling signal amplification and enabling precise visualization of the target antigen. Our secondary antibodies are conjugated with multiple labels, including Horseradish Peroxidase (HRP), Alkaline Phosphatase (AP), and fluorescent dyes, making them ideal for applications like chemiluminescent detection, fluorescence-based imaging, and colorimetric assays.

Our portfolio includes secondary antibodies suitable for Immunofluorescence (IF) and Flow Cytometry (FC), designed for multicolor panels and high-resolution imaging. For instance, our FITC, Alexa Fluor, and PE conjugated secondary antibodies allow researchers to visualize cellular markers with high sensitivity. With species-specific secondary antibodies, researchers can work with primary antibodies from a wide variety of host species, ensuring compatibility across diverse experimental models.

Secondary antibodies are particularly useful in Immunohistochemistry (IHC) for tissue and cell-based assays, where signal amplification is essential to detect low-abundance proteins. The versatility of secondary antibodies allows them to be used in conjunction with numerous detection systems, providing researchers with the flexibility needed to optimize experimental conditions for a variety of targets.

▌Control Antibodies

Control Antibodies are critical for validating the specificity of your results. These antibodies help to eliminate the possibility of non-specific binding and ensure that observed signals are due to the intended antigen-antibody interactions. At Nebulabio, we offer a wide range of Isotype Control Antibodies and Positive Control Antibodies for various applications.

Isotype Controls are matched to the primary antibody's isotype and are used to assess non-specific background binding. These controls are essential for confirming the specificity of antibody binding, especially when working with complex samples or tissues. On the other hand, Positive Controls are used to confirm that the experimental system is functioning properly, and the antibody has the capability to detect the target antigen. For example, our antibodies against β-Actin, GAPDH, or Histone H3 are commonly used as positive controls for Western Blotting (WB) and Immunohistochemistry (IHC).

In addition to isotype and positive controls, we provide a full range of Protein Controls, which are crucial for normalizing data in quantitative assays. These proteins, including β-Actin, GAPDH, and Tubulin, are often used to validate the consistency of protein loading or signal detection in experiments, ensuring that results are not skewed by sample variability.

▌A Comprehensive Solution for Your Research Needs

At Nebulabio, we are committed to providing the highest quality antibodies that meet the diverse needs of modern research. Our antibodies are not only rigorously validated for a wide range of applications but are also backed by expert technical support to help you design successful experiments. Whether you're working in basic research, drug development, or clinical diagnostics, our antibodies offer the precision and reliability required to advance your scientific discovery.

Additionally, if you require custom antibody solutions, Nebulabio offers antibody customization services to meet the specific needs of your research. Whether you need an antibody for a unique target or require specific conjugations and formats, our team of experts is ready to assist you in developing tailored antibodies for your experimental protocols. Please feel free to  contact us for more information on how we can help with custom antibody production.